Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/58348
Type: Artigo de periódico
Title: Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams
Author: Souza, CEA
Rego, JPA
Lobo, CH
Oliveira, JTA
Nogueira, FCS
Domont, GB
Fioramonte, M
Gozzo, FC
Moreno, FB
Monteiro-Moreira, ACO
Figueiredo, JR
Moura, AA
Abstract: The present study is focused on the proteome of reproductive tract fluids from tropically-adapted Santa Ines rams. Seminal plasma, cauda epididymal (CEF) and vesicular gland fluid (VGF) proteins were analyzed by 2-D electrophoresis and mass spectrometry. Seminal plasma maps contained 302 +/- 16 spots, within the 4-7 pH range. From these maps, 73 spots were identified, corresponding to 41 proteins. Ram Seminal Vesicle Proteins (RSVP) 14 and 22 kDa and bodhesins 1 and 2 represented the most abundant seminal components. Other seminal proteins included clusterin, angiotensin-converting enzyme, matrix metalloproteinase-2, tissue-inhibitor of metalloproteinase-2, plasma glutamate carboxypeptidase, albumin, lactoferrin, alpha enolase, peroxiredoxin, leucine aminopeptidase, beta-galactosidase, among others. Later, seminal plasma gels were run within narrow pH intervals (3.9-5.1; 4.7-5.9; 5.5-6.7), allowing the additional identification of 21 proteins not detected in 4-7 pH maps. Major proteins of CEF and VGF were albumin and transferrin, and RSVPs, respectively. Western blots confirmed that RSVPs were mainly present in VGF while bodhesins, in VGF and CEF. Based on RT-PCR, RSVP and bodhesin genes were primarily expressed in the vesicular glands. In summary, the reproductive tract fluids of Brazilian hairy rams contain several categories of proteins, with potential roles in sperm protection, capacitation, acrosome reaction and sperm-oocyte interaction. This article is part of a Special Issue entitled: Farm animal proteomics. (c) 2012 Elsevier B.V. All rights reserved.
Subject: Seminal plasma
Binder of Sperm Protein
Spermadhesin
Ram
Sperm
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.jprot.2012.05.039
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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