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Type: Artigo de periódico
Title: Proteome analysis of the plasma protein layer adsorbed to a rough titanium surface
Author: Dodo, CG
Senna, PM
Custodio, W
Leme, AFP
Cury, AAD
Abstract: In this study a label-free proteomic approach was used to investigate the composition of the layer of protein adsorbed to rough titanium (Ti) after exposure to human blood plasma. The influence of the protein layer on the surface free energy (SFE) of the Ti was evaluated by contact angle measurements. Ti discs were incubated with blood plasma for 180min at 37 degrees C, and the proteins recovered were subjected to liquid chromatography coupled to tandem mass spectrometry analysis. A total of 129 different peptides were identified and assigned to 25 distinct plasma proteins. The most abundant proteins were fibronectin, serum albumin, apolipoprotein A-I, and fibrinogen, comprising 74.54% of the total spectral counts. Moreover, the protein layer increased the SFE of the Ti (p<0.05). The layer adsorbed to the rough Ti surface was composed mainly of proteins related to cell adhesion, molecule transportation, and coagulation processes, creating a polar and hydrophilic interface for subsequent interactions with host cells.
Subject: titanium
surface properties
surface-free energy
protein adsorption
human blood plasma
Country: Inglaterra
Editor: Taylor & Francis Ltd
Rights: fechado
Identifier DOI: 10.1080/08927014.2013.787416
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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