Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/58340
Type: Artigo de periódico
Title: Proteome analysis of castor bean seeds
Author: Campos, FAP
Nogueira, FCS
Cardoso, KC
Costa, GCL
Del Bem, LEV
Domont, GB
Da Silva, MJ
Moreira, RC
Soares, AA
Juca, TL
Abstract: Castor bean (Ricinus communis L.) seeds serve as raw material for the production of nonedible oil used in medicine and industry, whereas the presence of allergenic and toxic proteins in the residue left after oil extraction precludes the use of this protein-rich by-product in animal feeding. To better understand the enzymes involved in the biosynthesis and deuradation of fatty acids and to identify proteins with toxic/anti-nutritional properties, exZ, extracts of developing and germinating seeds were prepared and prefractionated according to solubility properties of the proteins. An enriched plastid organelle fraction embracing mostly plastids and rnitochondria was also prepared. Two-dimensional electrophoresis (2DE) reference map,, of these fractions were obtained from which nearly 400 proteins were identified by matrix-assisted laser desorption ionization-time of flight-time of flight (MALDI-TOF-TOF) mass spectrometry after a search in a National Center for Biotechnology Information (NCBI) database and in an expressed sequence tag (EST) primary bank prepared from a cDNA library of developing seeds. These proteomics techniques resulted in the identification Of several classes of seed reserve proteins such as 2S albumins, legumin-like and seed storage proteins, as well as other proteins of' plastidial or mitochondrial functions and proteins involved in plant defense against biotic and abiotic stresses. It is expected that the collected data will facilitate the application of genetic techniques to improve the quality/profile of castor seed fatty acids, and pave the way for a rational approach to inactivate allergenic and toxic proteins, allowing the use of castor bean meal in animal feeding 11
Subject: castor bean
mass spectrometry
proteome analysis
Ricinus communis
seed proteins
two-dimensional electrophoresis
Country: EUA
Editor: Int Union Pure Applied Chemistry
Rights: aberto
Identifier DOI: 10.1351/PAC-CON-09-01-09
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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