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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleThyroid hormone increases plasma cholesteryl ester transfer protein activity and plasma high-density lipoprotein removal rate in transgenic micept_BR
dc.contributor.authorBerti, JApt_BR
dc.contributor.authorAmaral, MECpt_BR
dc.contributor.authorBoschero, ACpt_BR
dc.contributor.authorNunes, VSpt_BR
dc.contributor.authorHarada, LMpt_BR
dc.contributor.authorCastilho, LNpt_BR
dc.contributor.authorOliveira, HCFpt_BR
unicamp.authorState Univ Campinas, Inst Biol, Dept Physiol & Biophys, Campinas, SP, Brazil State Univ Campinas, Fac Med Sci, Dept Clin Pathol, Campinas, SP, Brazil Univ Sao Paulo, Sch Med, Lipid Lab, BR-05508 Sao Paulo, Brazilpt_BR
dc.subject.wosHepatic Triglyceride Lipasept_BR
dc.subject.wosScavenger Receptorpt_BR
dc.description.abstractThyroid dysfunction produces multiple alterations in plasma lipoprotein levels, including high-density lipoprotein (HDL). Cholesteryl ester transfer protein (CETP) and hepatic lipase (HL) are important proteins that modulate the metabolism of HDL. Thus, the effect of thyroid hormone on the activities of CETP and of HL was investigated using hypothyroid and hyperthyroid CETP transgenic (Tg) and nontransgenic (nTg) mice. Hyperthyroid Tg mice plasma lipoprotein (LP) profile analysis showed a significant increase in the very-low-density lipoprotein (VLDL) fraction (P < .001) and decrease in the HDL fraction (P < .005). whereas in the hypothyroid Tg mice an increase in low-density lipoprotein (LDL) was observed (P < .02). CETP activity was measured as the transfer of C-14-cholesteryl ester (CE) from labeled HDL to LDL by an isotopic assay indicative of mass. Hyperthyroid Tg mice had twice as much plasma CETP activity as compared with their controls, while in hypothyroid Tg mice plasma CETP activity did not change. The role of CETP in determining the changes in LP profile of hyperthyroid animals was confirmed by showing that nTg wild-type hyperthyroid and euthyroid mice exhibited the same percent cholesterol distribution in LP. Postheparin HL activity measured in hyperthyroid Tg mice was significantly reduced (P < .05). H-3-cholesteryl oleoyl ether (H-3-Cet)-HDL plasma fractional removal rate (FRR) was approximately 2-fold faster in the hyperthyroid Tg mice than in controls, but was not modified in hypothyroid animals. Tissue uptake of H-3-Cet was examined in 10 tissue samples: levels were significantly increased in skeletal muscle and decreased in small intestine in hyperthyroid Tg mice, and decreased in the small intestine of hypothyroid Tg mice. In conclusion, the excess of thyroid hormone accelerates HDL metabolism in CETP transgenic mice mainly due to an increase in plasma CETP activity and independently from the HL activity. Hypothyroid status did not change CETP activity and HDL metabolism. Copyright (C) 2001 by W.B. Saunders
dc.relation.ispartofMetabolism-clinical And Experimentalpt_BR
dc.relation.ispartofabbreviationMetab.-Clin. Exp.pt_BR
dc.publisherW B Saunders Copt_BR
dc.identifier.citationMetabolism-clinical And Experimental. W B Saunders Co, v. 50, n. 5, n. 530, n. 536, 2001.pt_BR
dc.sourceWeb of Sciencept_BR
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