Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/57720
Type: Artigo de periódico
Title: Molecular and cytogenetic characterization of an AT-rich satellite DNA family in Urvillea chacoensis Hunz. (Paullinieae, Sapindaceae)
Author: Urdampilleta, JD
de Souza, AP
Schneider, DRS
Vanzela, ALL
Ferrucci, MS
Martins, ERF
Abstract: Urvillea chacoensis is a climber with 2n = 22 and some terminal AT-rich heterochromatin blocks that differentiate it from other species of the genus. The AT-rich highly repeated satellite DNA was isolated from U. chacoensis by the digestion of total nuclear DNA with HindIII and XbaI and cloned in Escherichia coli. Satellite DNA structure and chromosomal distribution were investigated. DNA sequencing revealed that the repeat length of satDNA ranges between 721 and 728 bp, the percentage of AT-base pairs was about 72-73% and the studied clones showed an identity of 92.5-95.9%. Although this monomer has a tetranucleosomal size, direct imperfect repetitions of similar to 180 bp subdividing it in four nucleosomal subregions were observed. The results obtained with FISH indicate that this monomer usually appears distributed in the terminal regions of most chromosomes and is associated to heterochromatin blocks observed after DAPI staining. These observations are discussed in relation to the satellite DNA evolution and compared with other features observed in several plant groups.
Subject: DAPI
FISH
Heterochromatin
Karyotype
Satellite DNA
Urvillea
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s10709-008-9332-0
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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