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Type: Artigo de periódico
Title: Modulation of the epithelial barrier by dexamethasone and prolactin in cultured Madin-Darby canine kidney (MDCK) cells
Author: Peixoto, EBMI
Collares-Buzato, CB
Abstract: Glucocorticoids and prolactin (PRL) have a direct effect on the formation and maintenance of tight junctions (TJs) in cultured endothelial and mammary gland epithelial cells. In this work, we investigated the effect of a synthetic glucocorticoid dexamethasone (DEX) and PRL on the paracellular barrier function in MDCK renal epithelial cells. DEX (4 mu M) + PRL (2 mu g/ml) and DEX alone increased significantly the transepithelial electrical resistance after chronic treatment (4 days) of confluent MDCK monolayers or after 24 h treatment of subconfluent monolayers. Immunoblotting and immunocytochemistry revealed no changes in the expression and distribution of TJ-associated proteins occludin, ZO-1 and claudin-1 in confluent monolayers after hormone addition. However, a marked increase in junctional content for occludin and ZO-1 with no changes in their total expression was observed in subconfluent MDCK monolayers 24 h exposed to DEX or DEX + PRL. No change in cell proliferation/growth was detected at subconfluent conditions following hormone treatment. An increase in the total number of viable cells was observed only in confluent MDCK monolayers after exposure to DEX + PRL suggesting that the main effect of these hormones on already established barrier may be associated with the inhibition of cell death. In Conclusion, our data Suggest that these hormones (specially dexamethasone) have an effect on TJ structure and function only during the formation of MDCK epithelial barrier by probably modulating the localization, stability or assembly of TJ proteins to membrane sites of intercellular contact. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Subject: epithelial barrier
tight junction
MDCK cell line
junctional proteins
Country: Inglaterra
Editor: Academic Press Ltd Elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.cellbi.2005.08.004
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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