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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleModulation of mitochondrial activity by S-nitrosoglutathione reductase in Arabidopsis thaliana transgenic cell linespt_BR
dc.contributor.authorFrungillo, Lpt_BR
dc.contributor.authorde Oliveira, JFPpt_BR
dc.contributor.authorSaviani, EEpt_BR
dc.contributor.authorOliveira, HCpt_BR
dc.contributor.authorMartinez, MCpt_BR
dc.contributor.authorSalgado, Ipt_BR
unicamp.author.emailionesm@unicamp.brpt_BR
unicamp.authorFrungillo, Lucas Palamim de Oliveira, Jusceley Fatima Saviani, Elzira Elisabeth Oliveira, Halley Caixeta Salgado, Ione Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorCarmen Martinez, M. Univ Autonoma Barcelona, Fac Biociencies, Dept Bioquim & Biol Mol, E-08193 Barcelona, Spainpt_BR
dc.subjectGSNO reductasept_BR
dc.subjectNitric oxidept_BR
dc.subjectAlternative oxidasept_BR
dc.subjectUncoupling proteinpt_BR
dc.subjectRespiratory chainpt_BR
dc.subjectArabidopsis thalianapt_BR
dc.subject.wosDependent Formaldehyde Dehydrogenasept_BR
dc.subject.wosPotato-tuber Mitochondriapt_BR
dc.subject.wosNitric-oxidept_BR
dc.subject.wosAlternative Oxidasept_BR
dc.subject.wosPlant-mitochondriapt_BR
dc.subject.wosReversible Inhibitionpt_BR
dc.subject.wosNitrosative Stresspt_BR
dc.subject.wosDisease Resistancept_BR
dc.subject.wosOxidative Stresspt_BR
dc.subject.wosComplex-ipt_BR
dc.description.abstractThe enzyme S-nitrosoglutathione reductase (GSNOR) has an important role in the metabolism of S-nitrosothiols (SNO) and, consequently, in the modulation of nitric oxide (NO)-mediated processes. Although the mitochondrial electron transport chain is an important target of NO, the role of GSNOR in the functionality of plant mitochondria has not been addressed. Here, we measured SNO content and NO emission in Arabidopsis thaliana cell suspension cultures of wild-type (WT) and GSNOR overexpressing (GSNOR(OE)) or antisense (GSNOR(AS)) transgenic lines, grown under optimal conditions and under nutritional stress. Respiratory activity of isolated mitochondria and expression of genes encoding for mitochondrial proteins were also analyzed. Under optimal growth conditions, GSNOR(OE) had the lowest SNO and NO levels and GSNOR(AS) the highest, as expected by the GSNO-consuming activity of GSNOR. Under stress, this pattern was reversed. Analysis of oxygen uptake by isolated mitochondria showed that complex I and external NADH dehydrogenase activities were inhibited in GSNOR(OE) cells grown under nutritional stress. Moreover, GSNOR(OE) could not increase alternative oxidase (AOX) activity under nutritional stress. GSNOR(AS) showed constitutively high activity of external NADH dehydrogenase, and maintained the activity of the uncoupling protein (UCP) under stress. The alterations observed in mitochondrial protein activities were not strictly correlated to changes in gene expression, but instead seemed to be related with the SNO/NO content, suggesting a post-transcriptional regulation. Overall, our results highlight the importance of GSNOR in modulating SNO and NO homeostasis as well mitochondrial functionality, both under normal and adverse conditions in A. thaliana cells. (C) 2012 Elsevier B.V. All rights reserved.pt
dc.relation.ispartofBiochimica Et Biophysica Acta-bioenergeticspt_BR
dc.relation.ispartofabbreviationBiochim. Biophys. Acta-Bioenerg.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationMARpt_BR
dc.identifier.citationBiochimica Et Biophysica Acta-bioenergetics. Elsevier Science Bv, v. 1827, n. 3, n. 239, n. 247, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.volume1827pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage239pt_BR
dc.description.lastpage247pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn0005-2728pt_BR
dc.identifier.wosidWOS:000315249000002pt_BR
dc.identifier.doi10.1016/j.bbabio.2012.11.011pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipSpanish Ministry of Science and Innovation [BFU2010-15090]pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [08/11636-5]pt
dc.description.sponsordocumentnumberSpanish Ministry of Science and Innovation [BFU2010-15090]pt
dc.date.available2014-07-30T14:03:33Z
dc.date.available2015-11-26T17:39:38Z-
dc.date.accessioned2014-07-30T14:03:33Z
dc.date.accessioned2015-11-26T17:39:38Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T14:03:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:39:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57694
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57694-
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