Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/57691
Type: Artigo de periódico
Title: Modulation of hepatic microsomal triglyceride transfer protein (MTP) induced by S-nitroso-N-acetylcysteine in ob/ob mice
Author: Oliveira, CPMS
Alves, VAF
Lima, VMR
Stefano, JT
Debbas, V
Sa, SV
Wakamatsu, A
Correa-Giannella, ML
de Mello, ES
Havaki, S
Tiniakos, DG
Marinos, E
de Oliveira, MG
Giannella-Neto, D
Laurindo, FR
Caldwell, S
Carrilho, FJ
Abstract: We evaluated the effects of a potent NO donor, S-nitroso-N-acetylcysteine (SNAC), on microsomal triglyceride transfer protein (MTP) expression in ob/ob mice. NAFLD was induced in male ob/ob mice using a methionine-choline deficient diet (MCD) concomitantly with oral SNAC fed solution (n = 5) or vehicle (control; n = 5) by gavage daily for 4 weeks. Livers were collected for histology and for assessing MTP by RT-qPCR, Western blot, immunohistochemistry and immunogold electron microscopy analyses. Histological analysis showed diffuse macro and microvesicular steatosis, moderate hepatocellular ballooning and moderate inflammatory infiltrate in ob/ob mice fed the MCD diet. With SNAC, mice showed a marked reduction in liver steatosis (p < 0.01), in parenchymal inflammation (p = 0.02) and in MTP protein immunoexpression in zone III (p = 0.05). Moreover, SNAC caused reduction of MTP protein in Western blot analysis (p < 0.05). In contrast, MTP mRNA content was significantly higher (p < 0.05) in mice receiving SNAC. Immuno-electron microscopy showed MTP localized in the rough endoplasmic reticulum of hepatocytes in both treated and untreated groups. However with SNAC treatment, MTP was also observed surrounding fat globules. Histological improvement mediated by a nitric oxide donor is associated with significantly altered expression and distribution of MTP in this animal model of fatty liver disease. Further studies are in progress to examine possible mechanisms and to develop SNAC as a possible therapy for human fatty liver disease. (C) 2007 Elsevier Inc. All rights reserved.
Subject: NAFLD
NASH
MCD diet
MTP
S-nitroso-N-acetylcysteine
SNAC
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.bcp.2007.04.013
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000248160700011.pdf886.27 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.