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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleCytotoxic effect of the diterpene lactone dehydrocrotonin from Croton cajucara on human promyelocytic leukemia cellspt_BR
dc.contributor.authorFreire, ACGpt_BR
dc.contributor.authorMelo, PDpt_BR
dc.contributor.authorAoyama, Hpt_BR
dc.contributor.authorHaun, Mpt_BR
dc.contributor.authorDuran, Npt_BR
dc.contributor.authorFerreira, CVpt_BR
unicamp.authorUniv Estadual Campinas, Dept Bioquim, Inst Biol, UNICAMP, BR-13083970 Campinas, SP, Brazil State Univ Campinas, Inst Chem, Biol Chem Lab, UNICAMP, Campinas, SP, Brazilpt_BR
dc.subject.wosRapid Colorimetric Assaypt_BR
dc.subject.wosTrans-dehydrocrotoninpt_BR
dc.subject.wosGrowthpt_BR
dc.subject.wosSurvivalpt_BR
dc.subject.wosProteinpt_BR
dc.description.abstractDiterpenes exhibit potent antineoplastic properties against human and murine carcinoma cell lines. trans-Dehydrocrotonin from Croton cajucara, a Brazilian medicinal plant, is a nor-diterpene with antiulcerogenic activity. In this work, we examined the effect of trans-dehydrocrotonin (t-DCTN) on the vitality of HL60 cells by assessing the MTT reduction, protein content and phosphatase activity of these cells. Protein quantification indicated that t-DCTN reduced the number of cells with an IC50 of 500 muM; mitochondrial function (MTT reduction), was also inhibited (IC50 = 300 MM), when the cells were treated for 24 h. In contrast, when the cells were treated with this lactone in the initial plating and cultured for 96 h, t-DCTN was more toxic for all parameters analyzed: MTT and phosphatase activity (IC50 = 180 muM) and protein content (IC50 = 150 muM). The flavonoid utilized as positive control myricetin and the following IC50 values were obtained after 24 h of treatment: 300 and 192 muM for protein content and MTT reduction, respectively. According to the chemical characteristics of both compounds, the cytotoxic effect of t-DCTN could be explained through two mechanisms: adduct formation with DNA and proteins and/or oxidative stress induction.pt
dc.relation.ispartofPlanta Medicapt_BR
dc.relation.ispartofabbreviationPlanta Med.pt_BR
dc.publisher.cityStuttgartpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherGeorg Thieme Verlag Kgpt_BR
dc.date.issued2003pt_BR
dc.date.monthofcirculationJANpt_BR
dc.identifier.citationPlanta Medica. Georg Thieme Verlag Kg, v. 69, n. 1, n. 67, n. 69, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume69pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage67pt_BR
dc.description.lastpage69pt_BR
dc.rightsabertopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0032-0943pt_BR
dc.identifier.wosidWOS:000180899300013pt_BR
dc.identifier.doi10.1055/s-2003-37036pt_BR
dc.date.available2014-11-18T18:05:57Z
dc.date.available2015-11-26T17:53:02Z-
dc.date.accessioned2014-11-18T18:05:57Z
dc.date.accessioned2015-11-26T17:53:02Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T18:05:57Z (GMT). No. of bitstreams: 1 WOS000180899300013.pdf: 115394 bytes, checksum: 9dd35716ba857201a5918c7da79afa60 (MD5) Previous issue date: 2003en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:53:02Z (GMT). No. of bitstreams: 2 WOS000180899300013.pdf: 115394 bytes, checksum: 9dd35716ba857201a5918c7da79afa60 (MD5) WOS000180899300013.pdf.txt: 13091 bytes, checksum: a80575a1a5ade66d2a29452ff3ef8b49 (MD5) Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57628pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/57628
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57628-
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