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Type: Artigo de periódico
Title: Cytotoxic effect of the diterpene lactone dehydrocrotonin from Croton cajucara on human promyelocytic leukemia cells
Author: Freire, ACG
Melo, PD
Aoyama, H
Haun, M
Duran, N
Ferreira, CV
Abstract: Diterpenes exhibit potent antineoplastic properties against human and murine carcinoma cell lines. trans-Dehydrocrotonin from Croton cajucara, a Brazilian medicinal plant, is a nor-diterpene with antiulcerogenic activity. In this work, we examined the effect of trans-dehydrocrotonin (t-DCTN) on the vitality of HL60 cells by assessing the MTT reduction, protein content and phosphatase activity of these cells. Protein quantification indicated that t-DCTN reduced the number of cells with an IC50 of 500 muM; mitochondrial function (MTT reduction), was also inhibited (IC50 = 300 MM), when the cells were treated for 24 h. In contrast, when the cells were treated with this lactone in the initial plating and cultured for 96 h, t-DCTN was more toxic for all parameters analyzed: MTT and phosphatase activity (IC50 = 180 muM) and protein content (IC50 = 150 muM). The flavonoid utilized as positive control myricetin and the following IC50 values were obtained after 24 h of treatment: 300 and 192 muM for protein content and MTT reduction, respectively. According to the chemical characteristics of both compounds, the cytotoxic effect of t-DCTN could be explained through two mechanisms: adduct formation with DNA and proteins and/or oxidative stress induction.
Country: Alemanha
Editor: Georg Thieme Verlag Kg
Citation: Planta Medica. Georg Thieme Verlag Kg, v. 69, n. 1, n. 67, n. 69, 2003.
Rights: aberto
Identifier DOI: 10.1055/s-2003-37036
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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