Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/57505
Type: Artigo de periódico
Title: Cupin: A candidate molecular structure for the Nep1-like protein family
Author: Cechin, AL
Sinigaglia, M
Lemke, N
Echeverrigaray, S
Cabrera, OG
Pereira, GAG
Mombach, JCM
Abstract: Background: NEP1-like proteins (NLPs) are a novel family of microbial elicitors of plant necrosis. Some NLPs induce a hypersensitive-like response in dicot plants though the basis for this response remains unclear. In addition, the spatial structure and the role of these highly conserved proteins are not known. Results: We predict a 3d-structure for the beta-rich section of the NLPs based on alignments, prediction tools and molecular dynamics. We calculated a consensus sequence from 42 NLPs proteins, predicted its secondary structure and obtained a high quality alignment of this structure and conserved residues with the two Cupin superfamily motifs. The conserved sequence GHRHDWE and several common residues, especially some conserved histidines, in NLPs match closely the two cupin motifs. Besides other common residues shared by dicot Auxin-Binding Proteins (ABPs) and NLPs, an additional conserved histidine found in all dicot ABPs was also found in all NLPs at the same position. Conclusion: We propose that the necrosis inducing protein class belongs to the Cupin superfamily. Based on the 3d-structure, we are proposing some possible functions for the NLPs.
Country: Inglaterra
Editor: Biomed Central Ltd
Rights: aberto
Identifier DOI: 10.1186/1471-2229-8-50
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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