Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/57501
Type: Artigo de periódico
Title: CULTURE OF SOYBEAN FRUIT EXPLANTS - GROWTH-CONDITIONS AND EFFICIENCY OF NITROGEN-SOURCES FOR RESERVE PROTEIN-SYNTHESIS
Author: MOSQUIM, PR
SODEK, L
Abstract: A system was devised for the in vitro culture of soybean fruits. The culture svstem consisted of a single fruit attached to a short piece of stem through which the nutrients were supplied. The fruit explants were taken when pods were fully expanded and the seeds at initial stages of growth. During a 7-day culture period, the seeds accumulated dry matter and protein in quantities comparable to those in situ. Omission of the C source (sucrose) from the medium resulted in no dry matter accumulation in the seeds, but omission of the N source (glutamine) still led to some protein accumulation, indicating mobilization of N from other parts of the fruit explant. Optimum protein accumulation occurred when glutamine was supplied at 1.2 mg N ml-1. Protein accumulation in the seeds was highly dependent on the nature of the N source. Glutamine, asparagine and the ureide, allantoin, were equally the most efficient sources, whereas several other amino acids tested showed lower degrees of efficiency. The data indicate a high metabolic capacity of the fruit tissues for principal N transport compounds of soybean, namely allantoin, asparagine and glutamine. The culture system described should prove useful for developmental and metabolic studies where the complex influence of the rest of the plant is to be avoided.
Subject: GLYCINE-MAX
INVITRO FRUIT CULTURE
N-METABOLISM
Country: Holanda
Editor: Kluwer Academic Publ
Rights: fechado
Identifier DOI: 10.1007/BF00048209
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOSA1991GM46000012.pdf527.79 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.