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Type: Artigo de periódico
Title: Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica
Author: Marana, SR
Cancado, FC
Valerio, AA
Ferreira, C
Terra, WR
Barbosa, JARG
Abstract: Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstrom using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstrom, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstrom), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.
Country: Inglaterra
Editor: Blackwell Publishing
Rights: aberto
Identifier DOI: 10.1107/S174430910602401
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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