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|Type:||Artigo de periódico|
|Title:||CROTOXIN INDUCES AGGREGATION OF HUMAN WASHED PLATELETS|
|Abstract:||Crotoxin, the main toxic component isolated from the venom of the South American rattlesnake Crotalus durissus terrificus, is a reversible protein complex composed of a non-toxic non-enzymatic acidic polypeptide (crotapotin) and a toxic basic phospholipase A(2) (PLA(2)). In this study, we have evaluated the ability of crotoxin to induce aggregation in human washed platelets. Human washed platelet aggregation was monitored in a Payton aggregometer and thromboxane B-2 (TXB(2)) release measured by direct radioimmunoassay (RIA). Crotoxin (15-50 mu g/ml) produced dose-dependent and irreversible human washed platelet aggregation, which was inhibited by pre-incubation of the platelets with sodium nitroprusside (50-500 mu M) or iloprost (8-80 nM). Crotoxin also induced TXB, release (207 +/- 8 ng/ml, n = 6), and although indomethacin significantly reduced the release of TXB, (to 23.5 +/- 5 ng/ml, P < 0.001, n = 6), it did not inhibit crotoxin-induced aggregation. Our results clearly demonstrate that crotoxin induces human washed platelet aggregation and that this phenomenon is independent of the formation of pro-aggregatory arachidonic acid metabolites.|
|Editor:||Pergamon-elsevier Science Ltd|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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