Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/57426
Type: Artigo de periódico
Title: CROTOXIN INDUCES AGGREGATION OF HUMAN WASHED PLATELETS
Author: LANDUCCI, ECT
CONDINONETO, A
PEREZ, AC
HYSLOP, S
CORRADO, AP
NOVELLO, JC
MARANGONI, S
OLIVEIRA, B
ANTUNES, E
DENUCCI, G
Abstract: Crotoxin, the main toxic component isolated from the venom of the South American rattlesnake Crotalus durissus terrificus, is a reversible protein complex composed of a non-toxic non-enzymatic acidic polypeptide (crotapotin) and a toxic basic phospholipase A(2) (PLA(2)). In this study, we have evaluated the ability of crotoxin to induce aggregation in human washed platelets. Human washed platelet aggregation was monitored in a Payton aggregometer and thromboxane B-2 (TXB(2)) release measured by direct radioimmunoassay (RIA). Crotoxin (15-50 mu g/ml) produced dose-dependent and irreversible human washed platelet aggregation, which was inhibited by pre-incubation of the platelets with sodium nitroprusside (50-500 mu M) or iloprost (8-80 nM). Crotoxin also induced TXB, release (207 +/- 8 ng/ml, n = 6), and although indomethacin significantly reduced the release of TXB, (to 23.5 +/- 5 ng/ml, P < 0.001, n = 6), it did not inhibit crotoxin-induced aggregation. Our results clearly demonstrate that crotoxin induces human washed platelet aggregation and that this phenomenon is independent of the formation of pro-aggregatory arachidonic acid metabolites.
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/0041-0101(94)90111-2
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOSA1994MU93700009.pdf547.29 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.