Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Correlation between inflammatory cells and sulfated glycosaminoglycan concentration in synovial fluid of subjects with secondary knee osteoarthritis
Author: Franco, RN
Neto, PFAC
Pimentel, ER
Cohen, M
Lima, GEG
Mattiello-Rosa, SMG
Abstract: Objective. To analyze the presence of inflammatory cells in the synovial fluid (SF) of subjects with osteoarthritis (OA) and correlate them with the concentration of sulfated glycosaminoglycan (GAG). Methods. The degree of chondral injury from 20 subjects (17 men, 3 women, mean age 48.06 +/- 14.35 yrs) was evaluated macroscopically by means of arthroscopy and graded according to the International Cartilage Repair Society classification. All subjects presented anterior cruciate ligament (ACL) injuries, associated or not to menisci injuries. SF was aspirated and centrifuged. Sulfated GAG concentration was quantified by dimethylene blue staining. Manual morphometry was used to determine the inflammatory cell count (mononuclear and poly morphonuclear) by optical microscopy, after staining with May-Grunwald-Giemsa. The time of injury and degree of chondral injury were considered, and correlations among the variables were obtained by Spearman nonparametric correlation test. Results. There was no significant correlation between the amount of mononuclear cells and the GAG concentration. There was a significant positive correlation between the polymorphonuclear cells and the GAG concentration, and a significant negative correlation between the degree of chondral injury and the GAG concentration. Conclusion. Polymorphonuclear cells may be either a cause or an effect in the metabolic and biochemical processes occurring in chondral injuries. The correlations support the hypothesis that inflammatory cells have a significant role in the progression and chronicity of chondral injury in secondary OA.
Subject: osteoarthritis
sulfated glycosaminoglycans
mononuclear and polymorphonuclear leukocytes
Country: Canadá
Editor: J Rheumatol Publ Co
Citation: Journal Of Rheumatology. J Rheumatol Publ Co, v. 35, n. 6, n. 1096, n. 1101, 2008.
Rights: fechado
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000256503900027.pdf301.13 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.