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Type: Artigo de periódico
Title: Core-shell CdS/Cd(OH)(2) quantum dots: synthesis and bioconjugation to target red cells antigens
Author: De Farias, PMA
Santos, BS
De Menezes, FD
Ferreira, RD
Barjas-Castro, MD
Castro, V
Lima, PRM
Fontes, A
Cesar, CL
Abstract: We report a new and efficient methodology of labelling red blood cells, in order to investigate the expression of anti-A antigen, employing luminescent semiconductor nanocrystals. Highly luminescent and stable core-shell cadmium sulphide/cadmium hydroxide [CdS/CdS(OH)(2)] colloidal particles were obtained in the nanometre size range. The surface of these particles was characterized by using a monoclonal anti-A antibody via a one-step glutaraldehyde cross-linking procedure, followed by conjugation of the particles to red cells of blood groups A(+), A(2)(+) and O+. Laser scanning confocal microscopy images indicated that after conjugation for 30 min, A(+) and A(2)(+) erythrocytes presented different patterns of dual bright emission whereas the O+ group cells showed no emission. We suggest that this labelling procedure may be applied as a quantitative tool to investigate the distribution and expression of alloantigen in red blood cells.
Subject: bioconjugation
fluorescent markers
quantum dots
red blood cell
Country: Inglaterra
Editor: Blackwell Publishing
Rights: fechado
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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