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Type: Artigo de periódico
Title: Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae)
Author: Rey-Suarez, P
Floriano, RS
Rostelato-Ferreira, S
Saldarriaga-Cordoba, M
Nunez, V
Rodrigues-Simioni, L
Lomonte, B
Abstract: The major venom component of Micrurus mipartitus, a coral snake distributed from Nicaragua to northern South America, was characterized biochemically and functionally. This protein, named mipartoxin-I, is a novel member of the three-finger toxin superfamily, presenting the characteristic cysteine signature and amino acid sequence length of the short-chain, type-I, alpha-neurotoxins. Nevertheless, it varies considerably from related toxins, with a sequence identity not higher than 70% in a multiple alignment of 67 proteins within this family. Its observed molecular mass (7030.0) matches the value predicted by its amino acid sequence, indicating lack of post-translational modifications. Mipartoxin-I showed a potent lethal effect in mice (intraperitoneal median lethal dose: 0.06 mu g/g body weight), and caused a clear neuromuscular blockade on both avian and mouse nerve-muscle preparations, presenting a post-synaptic action through the cholinergic nicotinic receptor. Since mipartoxin-I is the most abundant (28%) protein in M. mipartitus venom, it should play a major role in its toxicity, and therefore represents an important target for developing a therapeutic antivenom, which is very scarce or even unavailable in the regions where this snake inhabits. The structural information here provided might help in the preparation of a synthetic or recombinant immunogen to overcome the limited venom availability. (C) 2012 Elsevier Ltd. All rights reserved.
Subject: Mipartoxin-I
Three-finger toxin
Micrurus mipartitus
Snake venom
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 60, n. 5, n. 851, n. 863, 2012.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2012.05.023
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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