Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/56978
Type: Artigo de periódico
Title: Milking and partial characterization of venom from the Brazilian spider Vitalius dubius (Theraphosidae)
Author: Rocha-e-Silva, TAA
Sutti, R
Hyslop, S
Abstract: The theraphosid spider genus Vitalius contains several species found in southeastern Brazil. In this work, we used electrostimulation to obtain venom from Vitalius dubius and examined its general composition. Male spiders yielded significantly less (p < 0.05) venom (12.5 +/- 0.7 mg of liquid/spider, n = 16: mean +/- S.E.M.) than female spiders (25.5 +/- 2.0 mg of liquid/spider, n = 11). However, when corrected for spider weight, males yielded slightly more venom (2.89 +/- 0.16 mg/g vs. 2.45 +/- 0.76 mg/g for males and females, respectively, p 0.05). Venom yield correlated linearly with spider weight for spiders weighing up to similar to 12-13 g, but decreased in very heavy females. There was a marked decrease in venom yield after the first milking. The protein concentration of pooled venom was 18.3 +/- 2.4 mg/ml (n = 4) and accounted for 16.6 +/- 4.7% of the dry venom weight. The venom contained high hyaluronidase activity (275 +/- 24 TRU/mg of protein, n = 4), with a molecular mass of similar to 45 kDa estimated by zymography. SDS-PAGE revealed a few proteins with molecular masses - 14 kDa but showed two staining bands of pepticles < 14 kDa. The venom reacted in ELISA with affinity-purified IgG from commercial arachnidic antivenom. Immunoblotting with this IgG detected proteins of 30-140 kDa only. Fractionation of the venom by reverse-phase chromatography resulted in five major and eight minor peaks. (c) 2008 Elsevier Ltd. All rights reserved.
Subject: Electrophoresis
Hyaluronidase
Sex variation
Theraphosidae
Venom milking
Vitalius dubius
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2008.10.026
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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