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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMicrosatellite loci for an endemic stingless bee Melipona seminigra merrillae (Apidae, Meliponini) from Amazonpt_BR
dc.contributor.authorFrancini, IBpt_BR
dc.contributor.authorSforca, DApt_BR
dc.contributor.authorSousa, ACBpt_BR
dc.contributor.authorCampos, Tpt_BR
dc.contributor.authorCidade, FWpt_BR
dc.contributor.authorZucchi, MIpt_BR
dc.contributor.authorSouza, APpt_BR
dc.contributor.authorNunes-Silva, CGpt_BR
dc.contributor.authorCarvalho-Zilse, GApt_BR
unicamp.author.emailibfrancini@yahoo.com.brpt_BR
unicamp.author.emailgislene@inpa.gov.brpt_BR
unicamp.authorFrancini, I. B. Nunes-Silva, C. G. Carvalho-Zilse, Gislene Almeida INPA, GPA, BR-69083060 Manaus, Amazonas, Brazilpt_BR
unicamp.authorSforca, D. A. Sousa, A. C. B. Campos, T. Cidade, F. W. Souza, A. P. Univ Estadual Campinas, Dept Genet & Evolucao, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorZucchi, M. I. Polo Apta Ctr, BR-13400970 Piracicaba, SP, Brazilpt_BR
unicamp.authorSouza, A. P. Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectAmazonian beespt_BR
dc.subjectConservationpt_BR
dc.subjectCross-amplificationpt_BR
dc.subjectMolecular markerspt_BR
dc.subjectGenetic diversitypt_BR
dc.subjectPollinatorspt_BR
dc.description.abstractMelipona seminigra merrillae is one of the main pollinators of Central Amazon native flora. These stingless bees are reared as economic alternative and for forest conservation purposes. Nine polymorphic microsatellite markers were characterized in 44 genotypes of M. seminigra merrillae from the Meliponary of the Instituto Nacional de Pesquisas da Amazonia, INPA (3 degrees 05.838'S 059 degrees 59.103'W), Manaus, Brazil. The number of alleles observed for each locus ranged from 3 to 8, with an average of 3.7 alleles per locus. The polymorphism information content (PIC) varied between 0.21 and 0.81 (average 0.50) and the discriminating power (D) ranged from 0.59 to 0.98 (average 0.77). The observed heterozygosity (H(o)) and the expected heterozygosity (H(e)) were 0.04-0.68 and 0.19-0.84, respectively. A cross-amplification test in three Melipona species suggested potential transferability of these microsatellites. These microsatellite markers should be useful for characterizing natural populations and specie conservation.pt
dc.relation.ispartofConservation Genetics Resourcespt_BR
dc.relation.ispartofabbreviationConserv. Genet. Resour.pt_BR
dc.publisher.cityDordrechtpt_BR
dc.publisher.countryHolandapt_BR
dc.publisherSpringerpt_BR
dc.date.issued2009pt_BR
dc.date.monthofcirculationDECpt_BR
dc.identifier.citationConservation Genetics Resources. Springer, v. 1, n. 1, n. 487, n. 490, 2009.pt_BR
dc.language.isoenpt_BR
dc.description.volume1pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage487pt_BR
dc.description.lastpage490pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0pt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1877-7252pt_BR
dc.identifier.wosidWOS:000207922400109pt_BR
dc.identifier.doi10.1007/s12686-009-9113-9pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2014-11-17T07:51:37Z
dc.date.available2015-11-26T16:41:18Z-
dc.date.accessioned2014-11-17T07:51:37Z
dc.date.accessioned2015-11-26T16:41:18Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:41:18Z (GMT). No. of bitstreams: 2 WOS000207922400109.pdf: 170207 bytes, checksum: d22206cddf6fa367ea5fdde0d22d20ce (MD5) WOS000207922400109.pdf.txt: 15388 bytes, checksum: a6cbbc6bfc1b32740d3509433557672e (MD5) Previous issue date: 2009en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56762pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/56762
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56762-
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