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Type: Artigo de periódico
Title: Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts
Author: Gunasekara, DB
Hulvey, MK
Lunte, SM
da Silva, JAF
Abstract: Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-l-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0 +/- 0.2). Nitrite was present in all samples tested.
Subject: Nitric oxide
Microchip electrophoresis
Reactive nitrogen species
Amperometric detection
Country: Alemanha
Editor: Springer Heidelberg
Rights: fechado
Identifier DOI: 10.1007/s00216-012-5810-4
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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