Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/56687
Type: Artigo de periódico
Title: Microbiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans
Author: Nucci, C
da Silveira, WD
Correa, SD
Nakazato, G
Bando, SY
Ribeiro, MA
de Castro, AFP
Abstract: It is difficult to use tissue culture assays to investigate adherence and other properties of Edwardsiella tarda because the organism is invasive and produces a potent hemolysin. We therefore relied on polymerase chain reaction (PCR) to determine the occurrence of genes for enterotoxins (LT-I, EAST-1), Shiga toxin (Stx-1, Stx-2), cytotoxic necrotizing factors (CNF-1, CNF-2), aerobactin, invasion plasmid of enteroinvasive Escherichia coli, EPEC adherence factor (EAF), intimin (Eae), enterohemolysin (EntHly) and hemolysin (lily) in 53 isolates of E. tarda from humans and fish from several countries. All isolates were negative for all genes investigated by PCR. Adhesion to and invasion of HeLa cells were determined by using the unusually short incubation time of 1 h or 30 min. All isolates adhered and invaded in these tests. Finally, a random amplified polymorphic DNA (RAPD) test distinguished, with a few exceptions, isolates of human and fish origin. (C) 2002 Elsevier Science B.V. All rights reserved.
Subject: Edwardsiella tarda
human and fish isolates
zoonoses
PCR
adhesion to HeLa
RAPD
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0378-1135(02)00151-7
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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