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Type: Artigo de periódico
Title: Methylation Pattern of THBS1, GATA-4, and HIC1 in Pediatric and Adult Patients Infected with Helicobacter pylori
Author: Alvarez, MC
Ladeira, MSP
Scaletsky, ICA
Pedrazzoli, J
Ribeiro, ML
Abstract: Background Helicobacter pylori infection is usually acquired in childhood and persists into adulthood if untreated. The bacterium induces a chronic inflammatory response, which is associated with epigenetic alterations in oncogenes, tumor-suppressor genes, cell-cycle regulators, and cell-adhesion molecules. Aim The aim of this study was to analyze the effect of H. pylori infection on the methylation status of Thrombospondin-1 (THBS1), Hypermethylated in cancer 1 (HIC1) and Gata binding protein-4 (GATA-4) in gastric biopsy samples from children and adults infected or uninfected with the bacterium and in samples obtained from gastric cancer patients. Methods The methylation pattern was analyzed with methylation-specific PCR. Results Our results showed that H. pylori infection was associated with methylation of the promoter regions of the THBS1 and GATA-4 genes in pediatric and adult samples (p < 0.01). HIC1 showed the lowest level of methylation, which was not an early event during gastric carcinogenesis. Conclusions The results from this study indicate that methylation of THBS1 and GATA-4 occurs in the early stages of chronic gastritis and gastric cancer in association with H. pylori infection; however, in gastric cancer samples, other mechanisms cooperate with the down-regulation of these genes. Methylation of HIC1 may not be the principal mechanism implicated in its down-regulation in gastric cancer samples.
Subject: Chronic gastritis
Gastric cancer
Helicobacter pylori
Country: Holanda
Editor: Springer
Citation: Digestive Diseases And Sciences. Springer, v. 58, n. 10, n. 2850, n. 2857, 2013.
Rights: fechado
Identifier DOI: 10.1007/s10620-013-2742-6
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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