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Type: Artigo de periódico
Title: Metalloproteinases 2 and-9 activity during promotion and progression stages of rat liver carcinogenesis
Author: Furtado, KS
Pires, PW
Justulin, LA
Rodrigues, MAM
Felisbino, SL
Barbisan, LF
Abstract: Activity of metalloproteinases 2 and 9 (MMP-2 and 9) during promotion and progression of rat liver carcinogenesis was investigated in a modified resistant hepatocyte model. Development of preneoplastic liver lesions positive for glutathione S-transferase 7-7-(GST-P 7-7-positive PNL) and tumors besides hepatocytes positive for proliferating cell nuclear antigen (PCNA) were quantified and compared to MMP-2 and-9 activity using gelatin zymography. Marked increases in GST-P 7-7-positive PNL development, PCNA labeling indices, MMP-2 (pro, intermediate and active forms) and pro-MMP-9 activity were observed after proliferative stimulus induced by 2-acetylaminofluorene (2-AAF) exposure cycles. After 2-AAF withdrawal, increase in MMP-2 activity was detected only in neoplastic mixed lesions, whereas active MMP-9 was increased in both PLN and neoplastic tissues. Our findings suggest that MMP-2 may be associated with proliferative events induced by 2-AAF rather than with selective growth of PNL and that MMP-9 could be associated with progression of PNL and neoplastic mixed lesions.
Subject: Rat liver carcinogenesis
Resistant hepatocyte model
Preneoplastic and neoplastic lesions
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s10735-008-9206-x
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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