Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/56256
Type: Artigo de periódico
Title: COMPARISON OF INDIRECT IMMUNOFLUORESCENCE WITH AGAR-GEL IMMUNODIFFUSION FOR THE DIAGNOSIS OF BLUETONGUE VIRUS-INFECTION
Author: ARITA, GMM
GATTI, MSV
GERMANO, PML
PESTANADECASTRO, AF
Abstract: 1. Sera from 190 cows and from 72 sheep were examined to compare the results obtained with the agar gel immunodiffusion (AGID) and indirect immunofluorescence (IIF) tests for the diagnosis of bluetongue (BT) disease. 2. In the AGID test, 96 of 190 (50.5%) cattle serum samples and 38 of 72 (52.7%) sheep serum samples were positive, for a total of 134 out of 262 (51.1%) sera. In the IIF test, 98 of 190 (51.6%) cattle serum samples and 39 of 72 (54.2%) sheep serum samples were positive, for a total of 137 out of 262 (52.3%) sera. 3. The fluorescence of the IIF test presented a granular cytoplasmic aspect, which in some cells was observed only on the cell membranes. 4. Statistical analysis of the data showed close agreement between the two techniques, regardless of the kind of sera examined. The IIF test showed high sensitivity (93.8% and 92.1%), specificity (91.4% and 88.2%) and positive (91.8% and 89.7%) and negative (93.48% and 90.9%) predictive values for cattle serum and sheep serum, respectively. 5. The results obtained with IIF were comparable to those obtained with the AGID test, indicating that both techniques can be used routinely in epidemiologic studies of BT. However, the IIF offers the additional advantages that it can be used for antibody quantification and for the detection of viral antigens in BT-infected cell lines.
Subject: BLUETONGUE VIRUS
IMMUNODIFFUSION TEST
INDIRECT IMMUNOFLUORESCENCE
Country: Brasil
Editor: Assoc Bras Divulg Cientifica
Rights: aberto
Date Issue: 1992
Appears in Collections:Unicamp - Artigos e Outros Documentos

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