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|Type:||Artigo de periódico|
|Title:||COMPARATIVE-EVALUATION OF 3 METHODS FOR THE DETERMINATION OF CHOLESTEROL IN EGG-YOLK|
|Abstract:||A comparative evaluation of three methods for the determination of cholesterol in egg yolk was conducted. The methods were: (a) the colorimetric method of Bohac et al. which involves a reaction with saturated acetic acid, ferrous sulfate and concentrated sulfuric acid; (b) the Adolfo Lutz Institute's method, also colorimetric, which utilizes glacial acetic acid, acetic anhydride and concentrated sulfuric acid; and (c) a high performance liquid chromatographic (HPLC) method, the conditions for which were established in the present work. For the latter method, a C-18 column was used, with acetonitrile-isopropanol (1:1) as mobile phase and a diode array detector; quantification was carried out by external standardization. The cholesterol concentrations obtained by the HPLC method (10.1 = 0.5 mg/g yolk) and that of Bohac et al. (11.0 +/- 1.0 mg/g yolk) did not differ significantly at 5% level. The colorimetric method of the Adolfo Lutz Institute overestimated the cholesterol contents (16.8 +/- 0.9 mg/g yolk).|
|Editor:||Inst Tecnologia Parana|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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