Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/56144
Type: Artigo de periódico
Title: COMPARATIVE STUDIES OF THE STABILITY OF FREE AND IMMOBILIZED INULINASE FROM Kluyveromyces marxianus NRRL Y-7571 IN AQUEOUS-ORGANIC SOLUTIONS
Author: Risso, FVA
Mazutti, MA
Costa, F
Treichel, H
Maugeri, F
Rodrigues, MI
Abstract: Enzymes have been extensively used in organic solvents to catalyze a variety of reactions of biological and industrial significance. In this work, the characteristics of free and immobilized inulinase were investigated in buffered solutions of butyl acetate. The influences of the organic solvent content on the optimal temperature and pH, the stabilities to temperature and pH and the kinetic parameters were systematically evaluated. The results showed that the organic solvent content had no effect on the optimal pH, either in the free or immobilized inulinase. For the immobilized enzyme, the optimal temperatures ranged from 55 degrees C to 60 degrees C, depending on the content of butyl acetate. At higher butyl acetate content, the stability of the immobilized enzyme increased for both pH and temperature. The organic solvent showed the tendency to increase the values of the kinetic parameters K-m and v(max) for both free and immobilized inulinase.
Subject: Organic solvent
Inulinase
Stability
Kinetic parameters
Country: Brasil
Editor: Brazilian Soc Chemical Eng
Rights: aberto
Identifier DOI: 10.1590/S0104-66322010000400002
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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