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Type: Artigo de periódico
Title: Cloning and Purification of IpaC Antigen from Shigella flexneri: Proposal of a New Methodology
Author: Mobilon, C
de Toledo, MAS
Paganelli, FL
dos Santos, CA
de Pace, F
de Paiva, JB
Stehling, EG
Nakazato, G
Balieiro, AG
Airoldi, FPD
Reis, FDM
da Silveira, WD
Abstract: Shigella flexneri is a Gram-negative bacillus that is responsible for a severe form of dysentery called Shigellosis, which mainly affects children and the elderly in both underdeveloped and developed countries. Pathogenic S. flexneri strains possess a large virulence plasmid that codes for effector proteins that are required for the entry and spread of the bacteria into colonocytes. Among these proteins is the translocator IpaC, which plays an important role in the invasion process; IpaC is implicated in pore formation in the host cell membrane and induces cytoskeletal rearrangements in macrophages and epithelial cells, thereby promoting bacterial entry. The ability of IpaC to insert into the plasma membrane is due to a large nonpolar region of the protein structure. This characteristic also renders difficulties in recovery and purification when the protein is expressed in E. coli. Several works have considered different methodologies for the improved production and purification of IpaC. Herein, we propose an alternative method that is based on changes in the induction temperature and extraction buffer to facilitate the accumulation of high yields of soluble proteins for their further processing and ultimate use in biotechnological approaches.
Subject: Shigella flexneri
IpaC production
Country: Emirados Árabes Unidos
Editor: Bentham Science Publ Ltd
Rights: fechado
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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