Please use this identifier to cite or link to this item:
|Type:||Artigo de periódico|
|Title:||Chromosome studies in Orchidaceae: karyotype divergence in Neotropical genera in subtribe Maxillariinae|
|Abstract:||Here, we study karyotype divergence in the closely related genera Brasiliorchis, Christensonella and Trigonidium belonging to subtribe Maxillariinae of subfamily Epidendroideae (Orchidaceae). We compare karyotypes in 15 species by (1) measuring 1C genome sizes, (2) mapping the distribution of 4',6-diamidino-2-phenylindole and chromomycin A3 chromosome bands and (3) localizing 5S and 45S nuclear ribosomal DNA (rDNA) sequences using fluorescent in situ hybridization. Recently, phylogenetic studies have been conducted to resolve species and genera relationships in subtribe Maxillariinae. We used these phylogenetic trees to map the cytogenetic characters in an evolutionary framework. This has enabled a better understanding of the patterns of genomic divergence in the group. Genome sizes range from 1C?=?1.85 to 4.1?pg. The largest, B.?schunkeana, shows evidence of genome upsizing, probably through the acquisition of tandem repeats that now form large 4',6-diamidino-2-phenylindole-positive blocks of heterochromatin. Our cytogenetic data are consistent with a base chromosome number of 2n?=?40, although Christensonella is characterized by a dysploid reduction in chromosome number to 2n?=?36. The number of 5S and 45S rDNA sites is variable between species, consistent with high rates of karyotype divergence. (c) 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 170, 29-39.|
in situ hybridization
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.