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dc.contributor.CRUESPUniversidade Estadual de Campinaspt
dc.typeArtigo de periódicopt
dc.titleChlorella modulates insulin signaling pathway and prevents high-fat diet-induced insulin resistance in micept
dc.contributor.authorVecina, JFpt
dc.contributor.authorOliveira, AGpt
dc.contributor.authorAraujo, TGpt
dc.contributor.authorBaggio, SRpt
dc.contributor.authorTorello, COpt
dc.contributor.authorSaad, MJApt
dc.contributor.authorQueiroz, MLDpt
unicamp.authorVecina, Juliana Falcato Torello, Cristiane Okuda de Souza Queiroz, Mary Luci Univ Estadual Campinas, Dept Pharmacol, Hemoctr, BR-13083970 Campinas, SP, Brazilpt
unicamp.authorOliveira, Alexandre Gabarra Araujo, Tiago Gomes Abdalla Saad, Mario Jose Univ Estadual Campinas, Dept Internal Med, BR-13081970 Campinas, SP, Brazilpt
unicamp.authorBaggio, Sueli Regina Food Technol Inst ITAL, Food Sci & Qual Ctr CCQA, BR-13070178 Campinas, SP, Brazilpt
dc.subjectChlorella vulgarispt
dc.subjectInsulin resistancept
dc.subjectInsulin signaling pathwaypt
dc.subject.wosGreen-algae Chlorellapt
dc.subject.wosCholesterol-fed Ratspt
dc.subject.wosHot-water Extractpt
dc.subject.wosLead-exposed Micept
dc.subject.wosVulgaris Extractpt
dc.subject.wosWistar Ratspt
dc.description.abstractAims: The search for natural agents that minimize obesity-associated disorders is receiving special attention. In this regard, the present study aimed to evaluate the prophylactic effect of Chlorella vulgaris (CV) on body weight, lipid profile, blood glucose and insulin signaling in liver, skeletal muscle and adipose tissue of diet-induced obese mice. Main methods: Balb/C mice were fed either with standard rodent chow diet or high-fat diet (HFD) and received concomitant treatment with CV for 12 consecutive weeks. Triglyceride, free fatty acid, total cholesterol and fractions of cholesterol were measured using commercial assay. Insulin and leptin levels were determined by enzyme-linked immunosorbent assay (ELISA). Insulin and glucose tolerance tests were performed. The expression and phosphorylation of IR beta, IRS-1 and Ala were determined by Western blot analyses. Key findings: Herein we demonstrate for the first time in the literature that prevention by CV of high-fat dietinduced insulin resistance in obese mice, as shown by increased glucose and insulin tolerance, is in part due to the improvement in the insulin signaling pathway at its main target tissues, by increasing the phosphorylation levels of proteins such as IR, IRS-1 and Akt. In parallel, the lower phosphorylation levels of IRS-lse' were observed in obese mice. We also found that CV administration prevents high-fat diet-induced dyslipidemia by reducing triglyceride, cholesterol and free fatty add levels. Significance: We propose that the modulatory effect of CV treatment preventing the deleterious effects induced by high-fat diet is a good indicator for its use as a prophylactic-therapeutic agent against obesity-related complications. (C) 2013 Elsevier Inc. All rights reserved.
dc.relation.ispartofLife Sciences
dc.publisherPergamon-elsevier Science Ltdpt
dc.identifier.citationLife Sciences. Pergamon-elsevier Science Ltd, v. 95, n. 1, n. 45, n. 52,
dc.sourceWeb of Sciencept
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsordocumentnumberFAPESP [10/50100-3, 11/50903-1]pt
dc.description.sponsordocumentnumberCNPq [304282/2011-1, 479854/2011-4]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T13:59:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:40:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
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