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Type: Artigo de periódico
Title: Biosensor for phenol based on the direct electron transfer blocking of peroxidase immobilising on silica-titanium
Author: Rosatto, SS
Kubota, LT
Neto, GD
Abstract: Horseradish peroxidase (HRP) was immobilised on silica gel modified with titanium oxide. This material was employed to prepare modified carbon paste electrode. The direct electron transfer of the hydrogen peroxide reduction by HRP was blocked when immobilised on silica-titanium, This biosensor presented a very sensitive response for phenol (1 mu mol l(-1)) at an applied potential of 0 mV vs SCE. The best condition was achieved in phosphate buffer pH 6.8, ratio of hydrogen peroxide/phenol higher than 0.35. The biosensor showed a linear response range between 10 and 50 mu mol l(-1) of phenol, adjusted by the equation j=-32.8+16.3 [phenol], for n=5 with a correlation coefficient of 0.9995. The response time of the biosensor was about 3 s. (C) 1999 Elsevier Science B.V. All rights reserved.
Subject: modified silica
phenol biosensor
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0003-2670(99)00168-3
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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