Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/55592
Type: Artigo de periódico
Title: Biological and enzymatic activities of Micrurus sp (Coral) snake venoms
Author: Cecchini, AL
Marcussi, S
Silveira, LB
Borja-Oliveira, CR
Rodrigues-Simioni, L
Amara, S
Stabeli, RG
Giglio, JR
Arantes, EC
Soares, AM
Abstract: The venoms of Micrurus lemniscatus carvalhoi, Micrurus frontalis frontalis, Micrurus surinamensis surinamensis and Micrurus nigrocinctus nigrocinctus were assayed for biological activities. Although showing similar liposome disrupting and myotoxic activities, M. frontalis frontalis and M. nigrocinctus nigrocinctus displayed higher anticoagulant and phospholipase A(2) (PLA(2)) activities. The latter induced a higher edema response within 30 min. Both venoms were the most toxic as well. In the isolated chick biventer cervicis preparation, M. lemniscatus carvalhoi venom blocked the indirectly elicited twitch-tension response (85+/-0.6% inhibition after a 15 min incubation at 5 mug of venom/mL) and the response to acetylcholine (ACh; 55 or 110 muM), without affecting the response to KCl (13.4 mM). In mouse phrenic nerve-diaphragm preparation, the venom (5 mug/mL) produced a complete inhibition of the indirectly elicited contractile response after 50 min incubation and did not affect the contractions elicited by direct stimulation. M. lemniscatus carvalhoi inhibited H-3-L-glutamate uptake in brain synaptosomes in a Ca2+, but not time dependent manner. The replacement of Ca2+ by Sr2+ and ethylene glycol-bis(beta-aminoethyl ether) (EGTA), or alkylation of the venom with p-bromophenacyl bromide (BPB), inhibited 3 H-L-glutamate uptake. M lemniscatus carvalhoi venom cross-reacted with postsynaptic alpha-neurotoxins short-chain (antineurotoxin-II) and long-chain (antibungarotoxin) antibodies. It also cross-reacted with antimyotoxic PLA(2) antibodies from M. nigrocinctus nigrocinctus (antinigroxin). Our results point to the need of catalytic activity for these venoms to exert their neurotoxic activity efficiently and to their components as attractive tools for the study of molecular targets on cell membranes. (C) 2004 Elsevier Inc. All rights reserved.
Subject: coral snake venoms
Micrurus sp.
Micrurus lemniscatus carvalhoi
liposome-disrupting activity
myotoxicity
neurotoxicity
phospholipase A(2)
synaptosome
L-glutamate uptake
Country: EUA
Editor: Elsevier Science Inc
Rights: fechado
Identifier DOI: 10.1016/j.cbpb.2004.11.012
Date Issue: 2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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