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Type: Artigo de periódico
Title: Biological activities of violacein, a new antitumoral indole derivative, in an inclusion complex with beta-cyclodextrin
Author: De Azevedo, MBM
Alderete, J
Rodriguez, JA
Souza, AO
Rettori, D
Torsoni, MA
Faljoni-Alario, A
Haun, M
Duran, N
Abstract: Violacein is a poorly water-soluble antitumoral and antibacterial drug. The solubility can be enhanced by complexation with beta-cyclodextrin. The inclusion complex was prepared by the co-precipitation method in molar ratios of 1 : 1 and 1 : 2 of violacein/beta-cyclodextrin, respectively. The acute toxicity (E. coli strain) of violacein did not change up to 400 mu M, either in the presence or absence of cyclodextrin. Cytotoxicity (V-79 cell culture) through DNA and MTT assays was significantly decreased in the presence of the 1 : 2 molar ratio complex. Studies on erythrocyte lipid peroxidation by the thiobarbituric acid (TBA) method showed that violacein and violacein/beta-CD (1 : 2) at 100 mu M cause 50% and 80% inhibition, respectively. At 500 mu M the violacein/beta-CD complex inhibited lipid peroxidation completely; however, with free violacein only 65% inhibition was reached at that concentration.
Subject: inclusion complex
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1023/A:1008138807481
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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