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Type: Artigo de periódico
Title: Biochemical characterization of a microbial glucosyltransferase that converts sucrose to palatinose
Author: Park, YK
Uekane, RT
Sato, HH
Abstract: A strain of Klebsiella sp. isolated from over-ripe fruits was shown to produce an intracellular glucosyltransferase that catalyses the conversion of sucrose to palatinose. The enzyme was purified by fractionation with ammonium sulfate, DEAE-Sephadex A-50 and CM-cellulose column chromatography. The purified enzyme was very active between pH 6.0 and 6.5 and the optimum temperature was 35 degrees C. The enzyme was inhibited by Hg2+ and Ag+ yet p-chloromercuribenzoate had no effect on enzyme activity. The Km and Vmax values for the purified enzyme were 120 mM and 110 mu g palatinose formed/, respectively. The molecular weight of the enzyme was 74,000 Da. The enzyme converted 4% sucrose (w/v) to isomaltulose after 64 hours incubation, with an efficiency of 86% at 25 degrees C, pH 6.5.
Subject: Klebsiella sp
palatinose production
sucrose transformation
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 1996
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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