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|Type:||Artigo de periódico|
|Title:||Biochemical characterization of a microbial glucosyltransferase that converts sucrose to palatinose|
|Abstract:||A strain of Klebsiella sp. isolated from over-ripe fruits was shown to produce an intracellular glucosyltransferase that catalyses the conversion of sucrose to palatinose. The enzyme was purified by fractionation with ammonium sulfate, DEAE-Sephadex A-50 and CM-cellulose column chromatography. The purified enzyme was very active between pH 6.0 and 6.5 and the optimum temperature was 35 degrees C. The enzyme was inhibited by Hg2+ and Ag+ yet p-chloromercuribenzoate had no effect on enzyme activity. The Km and Vmax values for the purified enzyme were 120 mM and 110 mu g palatinose formed/min.ml, respectively. The molecular weight of the enzyme was 74,000 Da. The enzyme converted 4% sucrose (w/v) to isomaltulose after 64 hours incubation, with an efficiency of 86% at 25 degrees C, pH 6.5.|
|Editor:||Soc Brasileira Microbiologia|
|Appears in Collections:||Artigos e Materiais de Revistas Científicas - Unicamp|
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