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Type: Artigo de periódico
Title: Biochemical Characterization and Pharmacological Properties of New Basic PLA(2) BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom
Author: Heleno, MAG
Baldasso, PA
Ponce-Soto, LA
Marangoni, S
Abstract: BrTX-I, a PLA(2), was purified from Bothrops roedingeri venom a. er only one chromatographic step using reverse-phase HPLC on mu-Bondapak C-18 column. A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues.. e total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLA(2) from snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA(2). BrTX-I presented PLA(2) activity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35-45 degrees C, and required Ca2+. In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNF alpha. were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively.
Country: EUA
Editor: Hindawi Publishing Corporation
Rights: aberto
Identifier DOI: 10.1155/2013/591470
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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