Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/55435
Type: Artigo de periódico
Title: Beta-lactamic Resistance Profiles in Porphyromonas, Prevotella, and Parvimonas Species Isolated from Acute Endodontic Infections
Author: Montagner, F
Jacinto, RC
Signoretti, FGC
de Mattos, VS
Grecca, FS
Gomes, BPFDA
Abstract: Introduction: Susceptibility to beta-lactamic agents has changed among anaerobic isolates from acute endodontic infections. The aim of the present study was to determine the prevalence of the cfxAlcfxA2 gene in Prevotella spp., Porphyromonas spp., and Parviomonas micra strains and show its phenotypic expression. Methods: Root canal samples from teeth with acute endodontic infections were collected and Porphyromonas, Prevotella, and Parvimonas micra strains were isolated and microbiologically identified with conventional culture techniques. The susceptibility of the isolates was determined by the minimum inhibitory concentration of benzylpenicillin, amoxicillin, and amoxicillin + clavulanate using the E-test method (AB BIODISK, Solna, Sweden). The presence of the cfxAlcfxA2 gene was determined through primer-specific polymerase chain reaction. The nitrocefin test was used to determine the expression of the lactamase enzyme. Results: Prevotella disiens, Prevotella oralis, Porphyromonas gin givalis, and P micra strains were susceptible to benzylpenicillin, amoxicillin, and amoxicillin + clavulanate. The cfxA/cfxA2 gene was detected in 2 of 29 isolates (6.9%). Simultaneous detection of the cfxAlcfxA2 gene and lactamase production was observed for 1 Prevotella buccalis strain. The gene was in 1 P micra strain but was not expressed. Three strains were positive for lactamase production, but the cfxAlcfxA2 gene was not detected through polymerase chain reaction. Conclusions: There is a low prevalence of the cfxAl cfxA2 gene and its expression in Porphyromonas spp., Prevotella spp., and P. micra strains isolated from acute endodontic infections. Genetic and phenotypic screening must be performed simultaneously to best describe additional mechanisms involved in lactamic resistance for strict anaerobes.
Subject: Anaerobic bacteria
antimicrobial agents
E-test
lactamases
nitrocefin
polymerase chain reaction
susceptibility
Country: EUA
Editor: Elsevier Science Inc
Rights: fechado
Identifier DOI: 10.1016/j.joen.2013.10.037
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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