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Type: Artigo de periódico
Title: Benefits of Blood Group Genotyping in Multi-Transfused Patients from the South of Brazil
Author: Guelsin, GAS
Sell, AM
Castilho, L
Masaki, VL
Melo, FC
Hashimoto, MN
Higa, TT
Hirle, LS
Visentainer, JEL
Abstract: We evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with hematological diseases and renal failure. Seventy-nine patients were selected. They all received more than three units of blood and eight (10%) had already clinical significant alloantibodies occurring alone or in combination against Rh, K, Fya, and Di antigens. DNA was prepared from blood samples and RHCE*E/e, KEL*01/KEL*02, FY*01/FY*02 and JK*01/JK*02 alleles were determined by using PCR-RFLP. RHD*/RHD*Psi and RHCE*C/c were tested using multiplex PCR. Discrepancies for Rh, Kell, Duffy, and Kidd systems were found between the phenotype and genotype-derived phenotype in 16 of the 38 chronically transfused patients. The genotypes of these patients were confirmed by DNA array analysis (HEA Beadchip (TM); Bioarray Solutions, Warren, NJ). Genotyping was very important for the determination of the true blood groups of the polytransfused patients, helped in the identification of suspected alloantibodies and in the selection of antigen- negative RBCs for transfusion. J. Clin. Lab. Anal. 24:311-316, 2010. (C) 2010 Wiley-Liss, Inc.
Subject: genotyping assays
blood group antigens
Country: EUA
Editor: Wiley-liss
Rights: fechado
Identifier DOI: 10.1002/jcla.20407
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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