Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/55375
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleBehavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranespt_BR
dc.contributor.authorBorsoi-Ribeiro, Mpt_BR
dc.contributor.authorBresolin, ITLpt_BR
dc.contributor.authorVijayalakshmi, Mpt_BR
dc.contributor.authorBueno, SMApt_BR
unicamp.author.emailsonia@feq.unicamp.brpt_BR
unicamp.authorBorsoi-Ribeiro, Mariana Lazzarotto Bresolin, Igor Tadeu Alves Bueno, Sonia Maria Univ Estadual Campinas, UNICAMP, Sch Chem Engn, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorLazzarotto Bresolin, Igor Tadeu Univ Fed Sao Paulo, UNIFESP, Dept Exact & Earth Sci, BR-09913030 Sao Paulo, Brazilpt_BR
unicamp.authorVijayalakshmi, Mookambeswaran Univ Technol Compiegne, Lab Interact Mol & Technol Separat, F-60205 Compiegne, Francept_BR
unicamp.authorVijayalakshmi, Mookambeswaran VIT Univ Vellore, Ctr Bioseparat Technol, Vellore 632014, Tamil Nadu, Indiapt_BR
dc.subjecthuman IgGpt_BR
dc.subjectIMACpt_BR
dc.subjectCu(II)pt_BR
dc.subjecttris(2-aminoethyl)aminept_BR
dc.subjectaffinity membranespt_BR
dc.subjectadsorptionpt_BR
dc.subject.wosAntibody Purificationpt_BR
dc.subject.wosHuman Serumpt_BR
dc.subject.wosMetal-ionspt_BR
dc.subject.wosMonoclonal-antibodypt_BR
dc.subject.wosL-histidinept_BR
dc.subject.wosChromatographypt_BR
dc.subject.wosProteinspt_BR
dc.subject.wosBindingpt_BR
dc.subject.wosFractionationpt_BR
dc.subject.wosSeparationpt_BR
dc.description.abstractIminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). The affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). The effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel. Copyright (c) 2013 John Wiley & Sons, Ltd.pt
dc.relation.ispartofJournal Of Molecular Recognitionpt_BR
dc.relation.ispartofabbreviationJ. Mol. Recognit.pt_BR
dc.publisher.cityHobokenpt_BR
dc.publisher.countryEUApt_BR
dc.publisherWiley-blackwellpt_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationOCTpt_BR
dc.identifier.citationJournal Of Molecular Recognition. Wiley-blackwell, v. 26, n. 10, n. 514, n. 520, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.volume26pt_BR
dc.description.issuenumber10pt_BR
dc.description.initialpage514pt_BR
dc.description.lastpage520pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0952-3499pt_BR
dc.identifier.wosidWOS:000323674900010pt_BR
dc.identifier.doi10.1002/jmr.2296pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-07-30T13:51:46Z
dc.date.available2015-11-26T16:41:06Z-
dc.date.accessioned2014-07-30T13:51:46Z
dc.date.accessioned2015-11-26T16:41:06Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T13:51:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:41:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55375
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55375-
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.