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Type: Artigo de periódico
Title: Bcl-2 prevents mitochondrial permeability transition and cytochrome c release via maintenance of reduced pyridine nucleotides
Author: Kowaltowski, A
Vercesi, AE
Fiskum, G
Abstract: Digitonin-permeabilized PC12 and GT1-7 neural cells exhibited a cyclosporin A-sensitive decrease in mitochondrial membrane potential, increased volume, and release of the proapoptotic factor cytochrome c in the presence of Ca2+ and the mitochondrial permeability transition (MPT) inducers t-butyl hydroperoxide (t-bOOH) or phenylarsine oxide (PhAsO). Although the concentration of PhAsO required to induce the MPT was similar for Bcl-2 negative and Bcl-2 overexpressing transfected cells (Bcl-2(+)), the level of t-bOOH necessary for triggering the MPT was much hi gh er for Bcl-2(+) cells, A higher concentration of t-bOOH was also necessary for promoting the oxidation of mitochondrial pyridine nucleotides in Bcl-2(+) cells. The sensitivity of Bcl-2(-)cell mitochondria to t-bOOH but not PhAsO could be overcome by the use of conditions that protect the pyridine nucleotides against oxidation, We conclude that the increased ability of Bcl-2(+) cells to maintain mitochondrial pyridine nucleotides in a reduced redox state is sufficient explanation for their resistance to MPT under conditions of oxidative stress induced by Ca2+ plus t-bOOH.
Subject: apoptosis
oxidative stress
pyridine nucleotides
Country: Inglaterra
Editor: Nature Publishing Group
Citation: Cell Death And Differentiation. Nature Publishing Group, v. 7, n. 10, n. 903, n. 910, 2000.
Rights: fechado
Identifier DOI: 10.1038/sj.cdd.4400722
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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