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Type: Artigo de periódico
Title: Autophagy is decreased in mesenteric fat tissue but not in intestinal mucosae of patients with Crohn's disease
Author: Leal, RF
Coy, CSR
Velloso, LA
Dalal, S
Portovedo, M
Rodrigues, VS
Coope, A
Ayrizono, MLS
Fagundes, JJ
Milanski, M
Abstract: Crohn's disease (CD) is a chronic intestinal disease with a multifactorial etiology. Recently, a role for mesenteric fat has been proposed in CD pathophysiology, since fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and the hypertrophied mesenteric tissue in CD. To evaluate autophagy-related proteins in intestinal mucosae and mesenteric fat of patients with CD and controls, patients with ileocecal CD (CD Group) and with non-inflammatory disease (FC Group) selected for surgery were studied. Expression of LC3-II was determined by immunoblotting of protein extracts. In addition, beclin-1, LC3 and Atg16-L1 RNA levels were measured using RT-PCR. The expression of LC3-II was significantly lower in the mesenteric tissue and higher in intestinal mucosae of CD when compared to controls. However, mRNA expression of autophagy-related proteins was similar when comparing the mesenteric fat groups. These findings suggest a defect in autophagy activation in the mesenteric fat tissue of CD individuals, which could be involved in the maintenance of the inflammatory process.
Subject: Crohn's disease
Inflammatory bowel disease
Mesenteric fat
Country: EUA
Editor: Springer
Citation: Cell And Tissue Research. Springer, v. 350, n. 3, n. 549, n. 552, 2012.
Rights: fechado
Identifier DOI: 10.1007/s00441-012-1491-8
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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