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Type: Artigo de periódico
Title: Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F-2 mice
Author: Valentinuzzi, VS
Kolker, DE
Vitaterna, MH
Shimomura, K
Whiteley, A
Low-Zeddies, S
Turek, FW
Ferrari, EAM
Paylor, R
Takahashi, JS
Abstract: The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical Limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F-2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test.
Country: EUA
Editor: Cold Spring Harbor Lab Press
Rights: embargo
Date Issue: 1998
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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