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Type: Artigo de periódico
Title: Association between high risk HPV viral load, p16(INK4A) expression and intra-epithelial cervical
Author: Eleuterio, J
Giraldo, PC
Cavalcante, DIM
Goncalves, AK
Eleuterio, RMN
Abstract: OBJECTIVE. To determine the (HR-HPV) high risk HPV viral load in squamous intra-epithelial lesions and association with p(16INK4A) expression. METHOS. A series of 109 cervical biopsies were studied (57 normal tissue, 26 low grade squamous intra-epithelial lesions [LSIL] and 26 high grade squamous intra-epithelial lesions [HSIL]). Detection of high risk HPV and viral load In cervical cells was made by molecular biology using hybrid capture 2nd generation collected before the biopsy. The p16(INK4a) was identified by immunohistochemistry using the p16(INK4a) kit (clone E6H4). RESULTs. High risk HPV was positive in 57.8% of all cases (29.8% in normal tissue, 80.8% In LSIL and 96.1% in HSIL). Protein p16(INK4a) was expressed in 23.8 % of squamous intra-epithelial lesions (15.4% In LSIL and 84.6961 in HSIL). In normal tissue all cases were negative to p16(INK4a). The viral load was higher in p16 positive cases than In negative cases (positive p16(INK4a) mean of 669.9 RLU/PCB [9.47 - 2814.9] and negative p16(INK4a) mean of 253.94 RLU/PCB [1.07 - 1882.21] (p<0.05). However when studying just the HSIL cases differences were not significant. CONCLUSION. In this study although the HR-HPV viral load had shown a significant difference between p16 positive and negative cases, In HSIL cases this finding was not confirmed, New studies with a larger number of cases are necessary for consistent conclusions.
Subject: HPV
cervical cancer
biomarkers of cervical cancer
viral load
Country: Brasil
Editor: Assoc Medica Brasileira
Rights: aberto
Identifier DOI: 10.1590/S0104-42302007000600022
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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