Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/54986
Type: Artigo de periódico
Title: Assessment of in situ butanol recovery by vacuum during acetone butanol ethanol (ABE) fermentation
Author: Mariano, AP
Qureshi, N
Maciel, R
Ezeji, TC
Abstract: BACKGROUND: Butanol fermentation is product limiting owing to butanol toxicity to microbial cells. Butanol (boiling point: 118 degrees C) boils at a higher temperature than water (boiling point: 100 degrees C) and application of vacuum technology to integrated acetonebutanolethanol (ABE) fermentation and recovery may have been ignored because of direct comparison of boiling points of water and butanol. This research investigated simultaneous ABE fermentation using Clostridium beijerinckii 8052 and in situ butanol recovery by vacuum. To facilitate ABE mass transfer and recovery at fermentation temperature, batch fermentation was conducted in triplicate at 35 degrees C in a 14 L bioreactor connected in series with a condensation system and vacuum pump. RESULTS: Concentration of ABE in the recovered stream was greater than that in the fermentation broth (from 15.7 g L-1 up to 33 g L-1). Integration of the vacuum with the bioreactor resulted in enhanced ABE productivity by 100% and complete utilization of glucose as opposed to a significant amount of residual glucose in the control batch fermentation. CONCLUSION: This research demonstrated that vacuum fermentation technology can be used for in situ butanol recovery during ABE fermentation and that C. beijerinckii 8052 can tolerate vacuum conditions, with no negative effect on cell growth and ABE production. (C) 2011 Society of Chemical Industry
Subject: acetone
butanol
ABE
Clostridium beijerinckii 8052
vacuum fermentation
integrated process
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1002/jctb.2717
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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