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Type: Artigo de periódico
Title: Assessment of fructooligosaccharides production from sucrose in aqueous and aqueous-organic systems using immobilized inulinase from Kluyveromyces marxianus NRRL Y-7571
Author: Risso, FVA
Mazutti, MA
Treichel, H
Costa, F
Maugeri, F
Rodrigues, MI
Abstract: This work investigated the fructooligosaccharides (FOS) synthesis by immobilized inulinase obtained from Kluyveromyces marxianus NRRL Y-7571 in aqueous and aqueous-organic systems using sucrose as substrate. The sequential strategy of experimental design was used to optimize the FOS conversion in both systems. For the aqueous-organic system, a 2(6-2) fractional design was carried out to evaluate the effects of temperature, sucrose concentration, pH, aqueous/organic ratio, enzyme activity, and polyethylene glycol concentration. For the aqueous system, a central composite design for the enzyme activity and the sucrose concentration was carried out. The highest fructooligosaccharides yield (Y-FOS) for the aqueous-organic system was 18.2 +/- S0.9 wt%, at 40 degrees C, pH 5.0, sucrose concentration of 60% (w/w), enzyme activity of 4 U.mL(-1) and aqueous/organic ratio of 25/75 wt%. The highest Y-FOS for the aqueous system was 14.6 +/- 0.9 wt% at 40 degrees C, pH 5.0, sucrose concentration of 60 wt%, and enzyme activity of 4.0 U.mL(-1).
Subject: immobilized inulinase
fructooligosaccharides synthesis
Kluyveromyces marxianus NRRL Y-7571
Country: Brasil
Editor: Soc Brasileira Ciencia Tecnologia Alimentos
Rights: aberto
Identifier DOI: 10.1590/S0101-20612012005000030
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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