Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/54930
Type: Artigo de periódico
Title: ASPARAGINASE FROM THE TESTA OF DEVELOPING LUPIN AND PEA-SEEDS
Author: SODEK, L
LEA, PJ
Abstract: Potassium independent asparaginase has been purified from the testa of maturing seeds of Lupinus polyphyllus. The enzyme has a M(r) of 71 x 10(3) and is composed of two subunits of M(r) 35-36 x 10(3). Antisera raised against the purified asparaginase cross-reacted with a protein of similar characteristics isolated from the cotyledons and leaves of L. polyphyllus but no reaction was detected with extracts from the roots and nodules following Western blot analysis. Potassium dependent asparaginase was purified to a lesser extent from the testa of pea seeds and was shown to have a M(r) of 61 x 10(3). The potassium independent and dependent enzymes appear to be distinct proteins with different immunological characteristics. The proportion of the two enzymes varies with plant species, organ and developmental age.
Subject: LUPINUS-POLYPHYLLUS
PISUM-SATIVUM
LEGUMINOSAE
ASPARAGINASE
IMMUNOLOGY
POTASSIUM
TESTA
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/S0031-9422(00)90781-7
Date Issue: 1993
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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