Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/549
Type: Artigo de periódico
Title: Isolation and Characterization of a Natriuretic Peptide from Crotalus oreganus abyssus (Grand Canyon Rattlesnake) and its Effects on Systemic Blood Pressure and Nitrite Levels
Author: SILVA, S. L. Da
ALMEIDA, J. R.
RESENDE, L. M.
MARTINS, W.
HENRIQUES, F. A. F. A.
BALDASSO, P. A.
SOARES, A. M.
TARANTO, A. G.
RESENDE, R. R.
MARANGONI, S.
DIAS-JUNIOR, C. A.
Abstract: Studies on the therapeutic potential of venom peptides have significantly advanced the development of new peptide drugs. A good example is captopril, a synthetic peptide drug, which acts as an anti-hypertensive and potentiating bradykinin, inhibiting the angiotensin-converting enzyme, whose precursor was isolated from the venom of Bothrops jararacussu. The natriuretic peptide (NPs) family comprises three members, ANP (atrial natriuretic peptide), BNP (B-type natriuretic peptide) and CNP (C-type natriuretic peptide), and has an important role in blood pressure regulation and electrolyte homeostasis. In this study, we describe, for the first time, the isolation and characterization of a novel natriuretic-like peptide (Coa_NP), isolated from Crotalus Oreganus abyssus venom. The peptide has 32 amino acids and its complete sequence is SKRLSNGCFGLKLDRIGAMSGLGCWRLINESK. The Coa_NP has an average molecular mass of 3510.98 Da and its amino acid sequence presents the loop region that is characteristic of natriuretic peptides (17 amino acids, NP domain consensus; CFGXXXDRIXXXSGLGC). Coa_NP is a natriuretic peptide of the ANP/BNP-like family, since the carboxy terminal region of CNP has its own NP domain. The functional experiments showed that Coa_NP produced biological effects similar to those of the other natriuretic peptides: (1) a dose-dependent decrease in mean arterial pressure; (2) significant increases in plasma nitrite levels, and (3) vasorelaxation in thoracic aortic rings that were pre-contracted with phenylephrine. The structural and biological aspects confirm Coa_NP as a natriuretic peptide isolated from snake venom, thus expanding the diversification of venom components.
Subject: Natriuretic peptide
Crotalus oreganus abyssus
Bioactive peptides
Nitric oxide
Country: Estados Unidos
Editor: SPRINGER
Citation: INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, v.17, n.3, p.165-173, 2011
Rights: fechado
Identifier DOI: 10.1007/s10989-011-9254-z
Address: http://dx.doi.org/10.1007/s10989-011-9254-z
http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000293236200002&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
Date Issue: 2011
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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