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Type: Artigo de periódico
Title: Aqueous Two-Phase Micellar System for Nisin Extraction in the Presence of Electrolytes
Author: Jozala, AF
Lopes, AM
Novaes, LCD
Mazzola, PG
Penna, TCV
Pessoa, A
Abstract: Liquid-liquid extraction for aqueous two-phase micellar systems (ATPMS) is a promising technique that can either replace or be used as a complementary process to more typical chromatographic operations in order to reduce the costs of downstream processing of many biological products. This method offers attractive conditions when exploited in the extraction/purification of a target protein directly on the culture medium. Nisin, an extracellular antimicrobial peptide, is produced by Lactococcus lactis and is effective at controlling a wide range of Gram-positive bacteria, including multidrug-resistant pathogens. This study evaluates ATPMS composed by a nonionic surfactant, Triton X-114, in the presence or absence of electrolytes, to improve the extraction of nisin. The partitioning behavior of nisin showed that it can be directly extracted from the fermentation media. The partitioning coefficient (K (nis)) of the commercial nisin in the presence of electrolytes showed K (nis) values of 5.6 and 5.4 for MgSO4 and (NH4)(2)SO4, respectively. Similar behavior was observed for the produced nisin where K (nis) values were 4.1 and 5.1 for MgSO4 and (NH4)(2)SO4. After partition, the commercial nisin activity in the micelle-rich phase, in the absence of electrolytes, was 3.3 logAU/mL, and in the presence of MgSO4 and (NH4)(2)SO4, the activity was 5.0 logAU/mL. The produced nisin activity with and without MgSO4 was around 3.5 logAU/mL; however, in the presence of (NH4)(2)SO4, nisin activity was 4.5 logAU/mL. The increase in nisin activity after partitioning with salts encourages further researches for the optimization of nisin extraction.
Subject: Liquid-liquid extraction
Lactococcus lactis
Peptide purification
Triton X-114
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s11947-012-1008-1
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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