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Type: Artigo de periódico
Title: Anti-sera raised in rabbits against crotoxin and phospholipase A(2) from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin
Author: Beghini, DG
Hernandez-Oliveira, S
Rodrigues-Simioni, L
Novello, JC
Hyslop, S
Marangoni, S
Abstract: Crotoxin, the principal neurotoxin in venom of the South American rattlesnakes Crotalus durissus terrificus and Crotalus durissus cascavella, contains a basic phospholipase A(2) (PLA(2)) and an acidic protein, crotapotin. In this work, we examined the ability of rabbit anti-sera against crotoxin and its PLA(2) subunit to neutralize the neurotoxicity of venom and crotoxin from C. d. cascavella in mouse phrenic nerve-diaphragm and chick biventer cervicis preparations. Immunoblotting showed that the anti-sera recognized C. d. cascavella crotoxin and PLA(2). This was confirmed by ELISA, with both anti-sera having end-point dilutions of 3 x 10(-6). Anti-crotoxin serum neutralized the neuromuscular blockade in phrenic nerve-diaphragm muscle preparations at venom or crotoxin:anti-serum ratios of 1:2 and 1:3, respectively. Anti-PLA(2) serum also neutralized this neuromuscular activity at a venom or crotoxin:anti-serum ratio of 1:1. In biventer cervicis preparations, the corresponding ratio for anti-crotoxin serum was 1:3 for venom and crotoxin, and 1:1 and 1:2 for anti-PLA(2) serum. The neutralizing capacity of the sera in mouse preparations was comparable to that of commercial anti-serum raised against C. d. terrificus venom. These results show that anti-sera against crotoxin and PLA(2) from C. d. cascavella venom neutralized the neuromuscular blockade induced by venom and crotoxin in both nerve-muscle preparations, with the anti-serum against crotoxin being slightly less potent than that against crotoxin. (C) 2004 Elsevier Ltd. All rights reserved.
Subject: anti-serum
neuromuscular blockade
phospholipase A(2)
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 44, n. 2, n. 141, n. 148, 2004.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2004.05.011
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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