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Type: Artigo de periódico
Title: Analysis of Soluble Lignin in Sugarcane by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with a Do-It-Yourself Oligomer Database
Author: Kiyota, E
Mazzafera, P
Sawaya, ACHF
Abstract: Lignin is a polymer found in the cell wall of plants and is one of the main obstacles to the implementation of second-generation ethanol production because it confers the recalcitrance of the lignocellulosic material. The recalcitrance of biomass is affected by the amount of lignin, by its monomer composition, and the way the monomers are arranged in the plant cell wall. Analysis of lignin structure demands mass spectrometry analysis, and identification of oligomers is usually based on libraries produced by laborious protocols. A robust method to build a do-it-yourself lignin oligomer library was tested. This library can be built using commercially available enzymes, standards, and reagents and is relatively easy to accomplish. An ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the separation and characterization of monomers and oligomers was developed and was equally applicable to the synthetic lignin and to soluble lignin extracted from a sample of sugar cane.
Country: EUA
Editor: Amer Chemical Soc
Rights: fechado
Identifier DOI: 10.1021/ac301112y
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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