Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/54567
Type: Artigo de periódico
Title: ANALYSIS OF INDOLE-3-ACETIC-ACID METABOLITES FROM DALBERGIA-DOLICHOPETALA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY
Author: OSTIN, A
MONTEIRO, AM
CROZIER, A
JENSEN, E
SANDBERG, G
Abstract: A mixture of [2-C-14(1)] and [C-13(6)]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [C-14(1),C-13(6)]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-C-14(1)] or [C-13(6)]indole-3-acetic acid into any of these conjugated indoles.
Editor: Amer Soc Plant Physiologists
Rights: fechado
Identifier DOI: 10.1104/pp.100.1.63
Date Issue: 1992
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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