Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/54518
Type: Artigo de periódico
Title: Analyses of the development and glycoproteins present in the ovarian follicles of Poecilia vivipara (Cyprinodontiformes, Poeciliidae)
Author: Rocha, TL
Yamada, AT
Costa, RME
Saboia-Morais, SMT
Abstract: Rocha T.L., Yamada A.T., Mazaro e Costa R. & Saboia-Morais S.M.T. 2011. Analyses of the development and glycoproteins present in the ovarian follicles of Poecilia vivipara (Cyprinodontiformes, Poeciliidae). Pesquisa Veterinaria Brasileira 31(1):87-93. Laboratorio de Comportamento Celular, Departamento de Morfologia, Instituto de Ciencias Biologicas, Universidade Federal de Goias, Campus II, ICB IV, Cx. Postal 131, Goiania, GO 74001-970, Brazil. E-mail: simonesaboias@gmail.com The morphofunctional aspects of oogenesis of Poecilia vivipara were studied aiming to understand the reproductive biology and development of species with internal fertilization, particularly those belonging to the family Poeciliidae. The stages of gonadal maturation and follicular development were characterized using mesoscopic, histological, histochemical, and lectin cytochemical analyses. Through mesoscopic evaluation the ovarian development was classified in six phases of development: immature, in maturation I, in maturation II, mature I, mature II, and post-spawn. Based on microscopic examination of the ovaries, we identified the presence of oocytes types I and II during the previtellogenic phase and types III, IV, and V during the vitellogenic phase. As oogenesis proceeded the oocyte cytosol increased in volume and presented increased cytoplasmic granule accumulation, characterizing vitellogenesis. The zona radiata (ZR) increased in thickness and complexity, and the follicular epithelium, which was initially thin and consisting of pavimentous cells, in type III oocytes exhibited cubic simple cells. The histochemical and cytochemical analyses revealed alterations in the composition of the molecular structures that form the ovarian follicle throughout the gonadal development. Our study demonstrated differences in the female reproductive system among fish species with internal and external fertilization and we suggest P. vivipara can be used as experimental model to test environmental toxicity.
Subject: Ovarian development
protein
histology
reproduction
ovoviviparity
Country: Brasil
Editor: Revista Pesquisa Veterinaria Brasileira
Rights: aberto
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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